SPECTROSCOPIC ESTIMATION OF METOPROLOL AND AMLODIPINE FROM TABLET DOSAGE FORM BY MULTIWAVELENGTH LINEAR REGRESSION ANALYSIS
Mrs. Yachita Jokhi, Dr. Megha Shah, Ms. Raskar Pranjal
Laxminarayandev College of Pharmacy, Bharuch, Gujarat, IndiaABSTRACT: A straightforward, cheapest spectrophotometric technique has been developed to measure both Metoprolol (MET) and Amlodipine (AML) simultaneously in dosage and bulk forms. The technique allows for the collection of data at various wavelengths to produce linear graphs, which is used to calculate concentrations. The absorbance values of the two analytes were linear with the concentration at the wavelengths taken at 10 nm interval over the range of 230 -300 nm. The accuracy and the precision of the new approach demonstrated were very good (RSD ˂ 2%). Developed method was validated in terms of linearity, range, precision, accuracy. The suitability of all methods for quantitative determination of these compounds was confirmed by validation in accordance with the requirements of ICH Guidelines.
KEYWORDS: Spectrophotometry, Multi-wavelength, Regression analysis, Metoprolol, Amlodipine